This readme.txt file was generated on 2023-10-14 by Matthias Pilecky, PhD


GENERAL INFORMATION

1. Title of Dataset: Chironomids sustain dietary quality via fatty acid regulation regardless of trophic state or dissolved organic carbon


2. Author Information
	A. Principal Investigator Contact Information
		Name: Matthias Pilecky, PhD
		Institution: WasserCluster Lunz - GmbH
		Address: Dr. Carl-Kupelwieser-Promenade 5
		Email: matthias.pilecky@donau-uni.ac.at


3. Date of data collection (single date, range, approximate date): 

2022-05-01 - 2020-07-31


4. Geographic location of data collection:

All samples were collected across southern Finland.

Aitalampi	N=62.93757, E=29.47697
Haarajärvi	N=61.23041, E=25.18558
Halsjärvi	N=61.22634, E=25.13733
Harkkojärvi	N=62.95278, E=31.02050
Hattujärvi	N=62.94949, E=31.21091
Haukijärvi	N=61.22225, E=25.13814
Hautjärvi	N=61.20348, E=25.14360
Hirsikankaan Rasku	N=63.16376, E=30.63771
Hokajärvi	N=61.24281, E=25.10688
Horkkajärvi	N=61.21397, E=25.15820
Höytiäinen	N=62.78540, E=29.70770
Hukkalampi	N=63.21476, E=30.94059
Hulausjärvi	N=61.28299, E=23.73752
Iso Ruuhijärvi	N=61.22377, E=25.06442
Iso Saunajärvi	N=63.17951, E=30.69214
Iso Valkjärvi	N=61.18939, E=25.10612
Iso Piilo	N=63.16889, E=30.84022
Kaivoslampi	N=63.19159, E=30.65316
Kalaton Väärä	N=63.16710, E=30.78200
Kangas Piilo	N=63.18063, E=30.84543
Kermajärvi	N=62.43728, E=28.63848
Kirkas Viinijärvi	N=62.64088, E=29.41210
Koitere	N=63.05859, E=30.82941
Kuorinka	N=62.61311, E=29.40340
Kyynäröjärvi	N=61.12387, E=24.98573
Litmonlampi	N=63.18678, E=30.65995
Lohjanjärvi	N=60.26139, E=24.04982
Majajärvi	N=61.21511, E=25.13638
Mekrijärvi	N=62.76636, E=30.95350
Nikkilänlampi	N=63.17523, E=30.94953
Nimetön	N=61.22772, E=25.19271
Nuorajärvi	N=62.68494, E=31.13630
Ormajärvi	N=61.11982, E=24.98249
Pääjärvi	N=61.05139, E=25.04292
Pieni Kangaslampi	N=63.17581, E=30.67327
Pieni Saunajärvi	N=63.17842, E=30.68873
Pieni Venejärvi	N=63.18639, E=30.71958
Pieni Piilo	N=63.16947, E=30.84450
Pikku Hukka	N=63.08601, E=30.49955
Pyhäselkä	N=62.60658, E=29.71575
Raate	N=63.09569, E=30.49991
Rauanlampi	N=62.78758, E=29.50683
Ritojärvi	N=63.20833, E=30.61459
Suomunjärvi	N=63.12558, E=30.74577
Synkkä Rasku	N=63.17714, E=30.61120
Syrjänalunen	N=61.19369, E=25.14047
Tuusulanjärvi	N=60.40926, E=25.02098
Viinijärvi	N=62.80946, E=29.30648
Valkea Mäntyjärvi	N=63.20730, E=30.61255
Valkealampi	N=63.22926, E=30.89061
Valkea Mustajärvi	N=61.22120, E=25.11853
Valkea Väärä	N=63.16131, E=30.78370
Valkjärvi	N=61.02484, E=25.16472
Vähä Ruuhijärvi	N=61.13597, E=25.45555
Vesijärvi	N=61.16912, E=25.45476
Ylinen	N=62.59592, E=30.22510


5. Information about funding sources that supported the collection of the data: 

This work has been supported by the Austrian Science Fund (FWF; I-3855 project ‘AquaTerr’ to Martin J. Kainz) and the Research Council of Finland (grants #338261 and #346541 to Ursula Strandberg). Matthias Pilecky received financial support from UEF Water research to conduct the laboratory and field studies at the University of Eastern Finland.


DATA & FILE OVERVIEW

1. File List: 
Fin_FieldStudy.csv    (Isotopic Data from the FieldStudy)
Fin_Experiment.csv      (Isotopic Data from the LabStudy)


2. Relationship between files, if important: 
Field and Lab Study Data from:

Matthias Pilecky, Aatu Turunen, Mohammad S. Sohrabi, Sadikshya Ghimire, Timo Ilo, Petri Kesti, Anssi Vainikka, Simon Vitecek, Jarkko Akkanen, Sami J. Taipale, Kimmo K. Kahilainen, Martin J. Kainz and Ursula Strandberg
Chironomids sustain dietary quality via fatty acid regulation regardless of trophic state or dissolved organic carbon


3. Additional related data collected that was not included in the current data package: 

Fatty acid quantification data can be found as supplementary information in the publication above or upon request to the corresponding author



METHODOLOGICAL INFORMATION

1. Description of methods used for collection/generation of data: 
See: Matthias Pilecky, Aatu Turunen, Mohammad S. Sohrabi, Sadikshya Ghimire, Timo Ilo, Petri Kesti, Anssi Vainikka, Simon Vitecek, Jarkko Akkanen, Sami J. Taipale, Kimmo K. Kahilainen, Martin J. Kainz and Ursula Strandberg
Chironomids sustain dietary quality via fatty acid regulation regardless of trophic state or dissolved organic carbon

2. Methods for processing the data: 
See: Matthias Pilecky, Aatu Turunen, Mohammad S. Sohrabi, Sadikshya Ghimire, Timo Ilo, Petri Kesti, Anssi Vainikka, Simon Vitecek, Jarkko Akkanen, Sami J. Taipale, Kimmo K. Kahilainen, Martin J. Kainz and Ursula Strandberg
Chironomids sustain dietary quality via fatty acid regulation regardless of trophic state or dissolved organic carbon

3. Instrument- or software-specific information needed to interpret the data: 
Standard .csv reader

4. Standards and calibration information, if appropriate: 
USGS70, USGS71 and USGS72 (C20-Me with certified d2H and d13C values)

5. Environmental/experimental conditions: 
See: Matthias Pilecky, Aatu Turunen, Mohammad S. Sohrabi, Sadikshya Ghimire, Timo Ilo, Petri Kesti, Anssi Vainikka, Simon Vitecek, Jarkko Akkanen, Sami J. Taipale, Kimmo K. Kahilainen, Martin J. Kainz and Ursula Strandberg
Chironomids sustain dietary quality via fatty acid regulation regardless of trophic state or dissolved organic carbon

6. Describe any quality-assurance procedures performed on the data: 
See: Pilecky M., Winter K., Wassenaar L.I., and Kainz M.J. (2020). 
Compound-specific deuterium analysis of lipids: applications for trophic ecology. Rapid Com Mass Spectro 35(16): e9135.

7. People involved with sample collection, processing, analysis and/or submission: 
Matthias Pilecky, Aatu Turunen, Mohammad S. Sohrabi, Sadikshya Ghimire, Petri Kesti, Anssi Vainikka, Simon Vitecek, Jarkko Akkanen, Sami J. Taipale, Kimmo K. Kahilainen, Martin J. Kainz,
Timo Ilo, Antti Tauru, Ursula Strandberg, Eszter Mergyeri and Maeliss Courtiere 


DATA-SPECIFIC INFORMATION FOR: Fin_FieldStudy.csv

1. Number of variables: 60

2. Number of cases/rows: 174

3. Variable List: 
Lake... Lake Name
Time... Month of sampling
State... Larvae or Imago
Region/Subregion/Area... geographical description
ID... Sample ID
14/14.Me13/.../14.1.5c/18.1.6c/BFA... Fatty acid mass fractions of chironomids [µg/mg]
TN... Total Nitrogen [µg/L]
TP... Total Phosphorus [µg/L]
DOC... Dissolved organic carbon [mg/L]
TFA... terrestrial fatty acids [µg/mg]
rAE... ratio of ARA (20:4n-6) to EPA (20:5n-3)
16:0_d13C/.../EPA_d13C... d13C values of FA (vs. VPDB)
16:0_d2H/.../EPA_d2H... d2H values of FA (vs. VSMOW)


DATA-SPECIFIC INFORMATION FOR: Fin_Experiment.csv

1. Number of variables: 7

2. Number of cases/rows: 698

ID	Compound	d2H	Treatment	Type	Group	Deuterium	Diet	Water


3. Variable List: 
ID... Sample ID
Compound... Fatty acid compound
d2H... d2H value of Fatty acid compound
Treatment... Treatment ID
Type... Diet itself or chironomidae larvae
Group... Group Variable
Deuterium... Artificial D2O labelling in water (logical variable)
Diet... Type of diet fed (Spirulina, TetraMin, Nettle, or a mixture of those)
Water... Culture medium / Lake Kuorinka / Lake Rauanlampi